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- Challenges and Chances: A Review of the 1st Stem Cell Community Day
- Summertime, and the Livin’ Is Easy…
- Follow-on-Biologics – More than Simple Generics
- Bacteria Versus Body Cells: A 1:1 Tie
- Behind the Crime Scene: How Biological Traces Can Help to Convict Offenders
- Every 3 Seconds Someone in the World Is Affected by Alzheimer's
- HIV – It’s Still Not Under Control…
- How Many Will Be Convicted This Time?
- Malaria – the Battle is Not Lost
- Physicians on Standby: The Annual Flu Season Can Be Serious
- At the Forefront in Fighting Cancer
- Molecular Motors: Think Small and yet Smaller Again…
- Liquid Biopsy: Novel Methods May Ease Cancer Detection and Therapy
- They Are Invisible, Sneaky and Disgusting – But Today It’s Their Special Day!
- How Many Cells Are in Your Body? Probably More Than You Think!
- What You Need to Know about Antibiotic Resistance – Findings, Facts and Good Intentions
- Why Do Old Men Have Big Ears?
- The Condemned Live Longer: A Potential Paradigm Shift in Genetics
- From Research to Commerce
- Chronobiology – How the Cold Seasons Influence Our Biorhythms
- Taskforce Microbots: Targeted Treatment from Inside the Body
- Eyes on Cancer Therapy
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JP | JPY
Impact of the CO2 incubator on contamination
Lab Academy
This article was published first in "Inside Cell Culture" , the monthly newsletter for cell culture professionals. Find more interesting articles about CO2 incubators on our page "FAQs and material on CO2 incubators" .
Sterility of cultures is a continuous challenge in cell culture. Germs may be transferred involuntarily by many different means:
In addition to these factors, the incubator plays an important role as it may increase or decrease the risk of culture contamination. Even though incubators do not provide a “sterile” environment, one can reduce the risk of contamination when following the below rules:
Sterility of cultures is a continuous challenge in cell culture. Germs may be transferred involuntarily by many different means:
- Contaminated cell stocks
- Cross contamination of cell stocks in liquid nitrogen
- Errors in aseptic technique
- Lack of strict general hygiene
- Malfunction of equipment
- Non-sterile plastic ware, media and supplements
In addition to these factors, the incubator plays an important role as it may increase or decrease the risk of culture contamination. Even though incubators do not provide a “sterile” environment, one can reduce the risk of contamination when following the below rules:
- Remove contaminated cultures from the incubator immediately
- Keep door opening times as short as possible
- Use incubator with split inner doors
- Immediately clean any spills inside the incubator
- Exchange the water frequently with autoclaved, distilled water
- Clean the incubator thoroughly once a month using an appropriate disinfectant
- Unscrew ventilators and other parts (if your incubator is equipped with) that often hide fungi for cleaning
- When fungal contamination occurs check the incubator thoroughly
- If possible, regularly decontaminate/sterilize using the respective function after cleaning
- Use HEPA filters in the incubator gas inflow if necessary
- Avoid the use of internal, non-sterilizable HEPA-filters
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